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A chromosome-level, haplotype-resolved genome assembly of the impressed tortoise, Manouria impressa
bioRxiv · · Preprint · Open access
Wang, Li
Abstract ↗AI summary
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A chromosome-level, haplotype-resolved genome for the impressed tortoise Manouria impressa was assembled, placing 94.5% of sequence into 26 chromosomes (2n = 52).
- Why it matters: A major gap is the lack of a chromosome-level genome for the genus Manouria, limiting conservation genomics and research on how this largely fungivorous tortoise specializes in diet.
- What they did: Using 68.5 Gb PacBio HiFi reads (28x), 9.8 Gb Hi-C, and RNA-seq from one female, the team built a 2.42 Gb assembly (contig N50 86.9 Mb; scaffold N50 138.6 Mb) and phased two haplotypes.
- The result: Hi-C scaffolding with quantitative curation placed 94.5% into 26 chromosomes, yielding QV 67.9 and 99.3% complete vertebrate BUSCOs, enabling a first chromosome-level genomic resource for Manouria.
The findingWhy it mattersWhat they didThe result
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