bioRxivJClub
Glycolytic enzymes form membrane-less condensates in the malaria parasite Plasmodium falciparum by sensing glucose levels
bioRxiv · · Preprint · Open access
Ishii, Sakura + 2 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
In Plasmodium falciparum asexual blood stage cells, low-glucose long-term culture drives formation of membrane-less condensates containing PFK9 and PGK.
- Why it matters: The parasite’s ABS relies on glycolysis for energy, but it was unknown whether glycolytic enzyme condensates form during its lifecycle or how they respond to host glucose reduction.
- What they did: Using fluorescence tagging and live imaging, the study tracked PFK9 and PGK condensate formation after long-term culture under low glucose, then tested membrane association with membrane staining and osmotic stress.
- The result: Condensates were stable yet not lipid-membrane associated, and PFK9 and PGK formed spatially distinct, non-overlapping structures, implying an enzyme-specific response to low glucose during ABS.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.
- Open access