bioRxivJClub
BlastPhyMe: A toolkit for rapid generation and analysis of protein-coding sequence datasets
bioRxiv · · Preprint · Open access
Gow, Ballesteros + 2 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
BlastPhyMe is a GUI-based toolkit that rapidly builds protein-coding sequence datasets and runs codon selection analyses, automating BLAST retrieval, alignment, phylogenies, and model testing.
- Why it matters: Building and analyzing protein-coding datasets requires many repetitive steps across multiple tools, creating time and usability barriers for researchers.
- What they did: It uses a portable database framework plus two modules: dataset assembly via BLAST with automatic coding-sequence extraction and taxonomic sorting, and selection analyses using codon-based likelihood methods through codeml (PAML) with automated replicate runs and likelihood ratio tests.
- The result: By saving alignments, trees, and results in the database and exporting publication-ready Excel tables, it enables faster, repeatable dataset generation and statistical model comparison.
The findingWhy it mattersWhat they didThe result
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- Open access
- 7 cites