An integrative single-cell epigenomic atlas annotates the regulatory genome of the adult mouse brain.

An integrative single-cell epigenomic atlas of the adult mouse brain assigns active, primed, and repressive chromatin states across 81% of the genome.

• Why it matters: Brain cell diversity depends on cell-type-specific regulatory programs, but a unified, multimodal map linking chromatin states to regulatory elements and repression modes has been missing.
• What they did: The study jointly profiled four histone modifications and transcriptomes in 2.5 million adult mouse brain nuclei, integrating chromatin accessibility, DNA methylation, and 3D genome organization.
• The result: Combinatorial active histone marks better nominate candidate enhancers than accessibility alone, while H3K27me3- and H3K9me3-marked states separate repression modes; the atlas supports deep learning that predicts cell-type-specific epigenomic features and gene expression from DNA sequence.

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