The abstract is read at the publisher; the summary is JClub's.
A promoter-proximal “super pause” sequence in human genes potently induces RNA polymerase II pausing and traps a reversible single-nucleotide backtracked “sidetracked” state.
- Why it matters: Pausing control depends on how DNA sequence and protein factors shape RNA polymerase II behavior, but the sequence-level mechanisms and offline states remain incompletely defined.
- What they did: The authors built GATO-seq for gene-specific, temporally resolved, reconstituted transcription using direct RNA sequencing of 3' ends, then solved cryo-EM structures on the super-pause sequence.
- The result: The super-pause–encoded sidetracked state is stabilized by a threonine-lined pocket and is not relieved by TF IIS, enabling in vitro dissection of sequence-encoded pausing propensity.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.