Virus-like particles enable highly efficient gene editing across porcine and avian in vitro, ex vivo, and in ovo models.

Virus-like particles enable highly efficient Cre recombination and CRISPR/Cas9 editing in porcine cells, organoids, and oocytes, especially under multiplexed delivery.

• Why it matters: Efficient delivery of genome editing tools remains a major bottleneck in porcine and avian livestock models, limiting functional genomics and translational research.
• What they did: The study tested VLPs as delivery vehicles for genome engineering in porcine and avian cultures, including in vitro cells, organoids, tracheal organ cultures, and in ovo embryos.
• The result: VLPs supported robust Cre recombination and Cas9-mediated editing across both species, with reporter and dCas9 VLPs showing platform versatility for livestock gene editing.

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