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Full-length RNA sequencing profiles intracellular and extracellular RNAs in euploid and aneuploid human embryos.
Nature Cell Biology · · Journal Article
Sugimoto, Mariya + more
Abstract ↗AI summary
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Aneuploid human blastocysts produce extracellular cfRNA profiles that differ from euploid embryos, showing stress-associated signatures including nonsense-mediated decay and TP53-mediated apoptosis.
- Why it matters: Cell-free RNA in embryo culture medium could report preimplantation development, but its source and how chromosomal status regulates it are unclear.
- What they did: Full-length total RNA sequencing was performed on individual human blastocysts and matched spent media, with stable gene and long non-coding RNA–mRNA co-expression modules linked to RNA processing, export, vesicle trafficking, autophagy, and degradation pathways.
- The result: Mouse perturbation experiments supported a link between autophagy–lysosome–vesicle pathway activity and embryo-derived cfRNA signatures, enabling biologically informative readouts of embryonic state.
The findingWhy it mattersWhat they didThe result
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