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E2F1 induces a G0-G1 reentry transcriptional program without changing chromatin accessibility.
EMBO Journal · · Journal Article · Open access
Lodewijk, Topacio + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Inducible E2F1 expression drives exit from quiescence and a G0–G1 reentry transcriptional program without altering chromatin accessibility in quiescent cells.
- Why it matters: Quiescent cells keep cell-cycle genes repressed through chromatin mechanisms, yet must remain poised to reenter the cycle; how E2F1 overcomes this block is unclear.
- What they did: The authors used time-resolved transcriptomics to compare E2F1 induction with serum stimulation, plus ChIP-seq for E2F1 binding in quiescent cells and biochemical reconstitution showing nucleosome-associated DNA access.
- The result: E2F1 activates canonical cell-cycle genes via limited-access chromatin and nucleosome-bound DNA, implying transcription can be initiated without major chromatin reorganization.
The findingWhy it mattersWhat they didThe result
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- Open access
- 1 cites