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Phospholipase D1 activates NAD kinase fuelling neutrophil extracellular trap formation.
Nature Communications · · Journal Article · Open access
Albarghash, San-Miguel + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Phospholipase D1 activation in human neutrophils is linked to NAD kinase activation, driving NADP⁺ production that supports NADPH oxidase-dependent NET formation.
- Why it matters: NADP⁺ availability during neutrophil activation is unclear, yet NET formation relies on NADPH oxidase-derived reactive oxygen species.
- What they did: Using integrated lipidomics, biochemistry, and functional assays in human neutrophils, the study shows phospholipase D1 generates phosphatidic acid and phosphatidylinositol 4,5-bisphosphate that bind and activate NAD kinase.
- The result: Pharmacologic or genetic phospholipase D1 disruption suppresses NAD kinase activity, depletes NADP⁺, and impairs NET formation independently of direct NADPH oxidase inhibition.
The findingWhy it mattersWhat they didThe result
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