The abstract is read at the publisher; the summary is JClub's.
A tethered reporter screen of 730 RNA-binding proteins found 281 RBPs that significantly modulate circular RNA activity, mapping trans-acting control across circRNA metabolism stages.
- Why it matters: Trans-acting factors that govern circRNA metabolism are poorly defined, limiting understanding of circRNA regulation and the ability to engineer circRNAs for RNA therapeutics.
- What they did: The study used a tethered reporter assay to test 730 RBPs, then followed up to assess effects on biogenesis, nuclear export, degradation, and translation; it also evaluated tethered translation enhancement for therapeutic circRNA vectors.
- The result: Seven RBPs enhanced tethered translation of therapeutically applicable circRNA vectors, with LARP4B strongly boosting exogenous circRNA translation across multiple internal ribosomal entry sites and cell types; RBM15 showed conserved roles in circRNA biogenesis and nuclear retention in fly, mouse, and human, and perturbing RBM15 sites with dCasRx or antisense oligonucleotides modulated endogenous circRNA expression.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.