Generation of four human induced pluripotent stem cell lines from dermal fibroblasts of healthy female donor.

Four integration-free human induced pluripotent stem cell lines were generated from dermal fibroblasts of a healthy 39-year-old female donor, using episomal reprogramming.

• Why it matters: Reliable, genomically stable hiPSC lines from healthy donors are needed as starting material for downstream disease modeling and regenerative studies.
• What they did: Dermal fibroblasts (Lonza, CC-2511) were reprogrammed with episomal plasmid-based methods via nucleofection (two lines) or lipofection (two lines), followed by pluripotency and stability checks.
• The result: The resulting lines showed undifferentiated marker expression, trilineage differentiation capacity, and normal karyotypes, enabling their use as integration-free pluripotent models.

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