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The DUF2439/RADIF domain of ZGRF1 is a RAD51 interactor and facilitator.
Cell Reports · · Journal Article
Pade, Brozkova + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
The DUF2439 domain of human ZGRF1 binds RAD51 in vitro and is linked to MMC resistance, with DUF2439 required for ZGRF1 recruitment to MMC-induced foci.
- Why it matters: DSB repair by homologous recombination depends on RAD51, yet how ZGRF1 and RAD54B coordinate RAD51 at DNA lesions is unclear, limiting understanding of responses to DNA crosslinks and PARP inhibition.
- What they did: The study tested DUF2439–RAD51 interaction in vitro, assessed MMC resistance, measured ZGRF1 recruitment to MMC-induced foci, and evaluated homology-directed repair of a defined DSB using ZGRF1 and RAD54B perturbations.
- The result: ZGRF1 and RAD54B show synergistic facilitation of homology-directed repair, consistent with reduced survival of double-mutant cells after MMC or talazoparib, supporting a model where DUF2439 helps recruit these factors to lesions.
The findingWhy it mattersWhat they didThe result
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