The abstract is read at the publisher; the summary is JClub's.
A quantitative nanoparticle enrichment plus data-independent acquisition workflow enables deep proteomic profiling of 20 µl mouse serum, identifying 3,992 protein groups across ages 12–30 months.
- Why it matters: Circulating biomarkers are needed to measure biological aging in mice, but serum proteomics are constrained by low sample volume and technical depth.
- What they did: The study used nanoparticle-based enrichment with data-independent acquisition mass spectrometry in 30 mice (12, 24, 30 months) and added 1 µl serum for proximity extension assay cytokine quantification.
- The result: Coverage increased 3–6-fold with median coefficient of variation <10%, revealing age- and sex-specific protein changes and senescence-associated circulating proteins, while 43 cytokines were quantified by proximity extension assay but not detected by mass spectrometry.
The findingWhy it mattersWhat they didThe result
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