A single-cell comprehensive atlas of leukemia and its microenvironment.

A comprehensive single-cell atlas of acute myeloid leukemia (AML) and its microenvironment (CALM) integrates 73 AML samples and 9 healthy donors to map linked cellular states and clinical annotations.

• Why it matters: AML relapse and treatment failure are linked to shifting cellular programs and changing interactions in the leukemic ecosystem, but a unified, clinically annotated single-cell resource has been lacking.
• What they did: CALM combines single-cell profiling from 73 AML samples plus 9 healthy donors, bulk RNA-seq from 87 newly diagnosed patients, and 4 paired bone marrow–peripheral blood single-cell datasets, all linked to clinical and treatment-response annotations.
• The result: Persistent disease shows greater clonal expansion among T cells, and machine learning on conventional T cells yields a T-cell signature associated with AML and outcomes, validated in two cohorts via the CALM portal.

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