Plant Physiol BiochemJClub
Binding pocket optimization of Pueraria mirifica type II chalcone isomerase synergizing with a necrosis-attenuating host transcription factor for persistent daidzein biosynthesis.
Plant Physiology and Biochemistry · · Journal Article · Open access
Suntichaikamolkul, Sanachai + more
Abstract ↗AI summary
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An engineered Pueraria mirifica type II chalcone isomerase (PmCHI Type II) I195V variant increases isoliquiritigenin selectivity (ratio 1.63) and supports higher daidzein accumulation in Nicotiana benthamiana.
- Why it matters: Heterologous isoflavonoid reconstitution in non-legume plants is bottlenecked by inefficient enzymes and severe host tissue necrosis that limits persistent pathway operation.
- What they did: Using 500-ns molecular dynamics plus structure-guided mutagenesis, the I195V active-site variant was tested in vitro and integrated into a polycistronic, P2A-stacked pathway with NbMYB60 replacing Arabidopsis thaliana MYB12.
- The result: NbMYB60 attenuated necrosis and extended viability up to 9 days, yielding 1.5 mg/g DW daidzein at 6 days post-infiltration, with high-performing replicates up to 2.5 mg/g DW.
The findingWhy it mattersWhat they didThe result
- Open access