PyMYB106-PyLAC12 Modulates Callus Browning Through Phenylpropanoid-Flavonoid Metabolism in Pinus yunnanensis.

PyLAC12, regulated by PyMYB106, drives callus browning in Pinus yunnanensis by modulating phenylpropanoid-flavonoid metabolism, with overexpression increasing browning and knockout reducing it.

• Why it matters: Callus browning hampers in vitro culture of woody plants, especially conifers, limiting their propagation and genetic improvement efforts. Understanding its molecular basis can improve tissue culture success.
• What they did: Researchers used multi-omics integration, transgenic overexpression, and knockout experiments to identify PyLAC12 as a key driver of browning, and demonstrated that PyMYB106 directly regulates PyLAC12 by binding to its promoter.
• The result: The findings reveal that the PyMYB106-PyLAC12 module influences flavonoid-phenylpropanoid pathways, providing targets for genetic or biochemical strategies to mitigate callus browning in woody plant tissue culture.