A phosphoproteome atlas of human cell lines reveals the landscape of kinase activity.

A phosphoproteome atlas covering over 200,000 class I phosphosites across 33 human cell lines enables single-shot phosphoproteomics with 30-fold faster data processing.

• Why it matters: Detection sensitivity, incomplete coverage, and computational bottlenecks—especially for low-input experiments—limit comprehensive phosphoproteomics and slow mapping of kinase-driven signaling in cancer.
• What they did: The study built an empirical human phosphoproteome resource from 33 diverse cell lines, then used it to improve single-shot analysis and phosphosite localization from minimal inputs.
• The result: By integrating proteome and phosphoproteome data, it defines a combined kinase activity score (Cscore) that reveals cell line- and cancer-specific signaling vulnerabilities linked to drug sensitivity.

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