Integrative structural modelling reveals the human Mic60-Mic19 subcomplex as a diffusion barrier in mitochondria.

The human Mic60-Mic19 subcomplex forms a dynamic tetrameric structure that acts as a diffusion barrier at mitochondrial crista junctions, with a key role in mitochondrial regulation.

• Why it matters: Understanding how mitochondrial crista junctions control the exchange of proteins, metabolites, and ions is crucial for insights into mitochondrial function and health, yet the structural basis of this regulation remains unclear.
• What they did: The study used crystallography, molecular dynamics simulations, and cross-linking experiments to model and validate the architecture of the Mic60-Mic19 complex, revealing its dynamic, multidomain nature.
• The result: This integrative approach demonstrates that the Mic60-Mic19 complex functions as a flexible diffusion barrier, providing a foundation for further exploration of mitochondrial gating mechanisms and potential therapeutic targets.