Taxane-induced conformational changes in the microtubule lattice activate GEF-H1-dependent RhoA signalling.
Taxanes activate RhoA signalling by inducing conformational changes in microtubule lattice that release GEF-H1, with rapid effects observed in interphase cells.
- Why it matters: Understanding how taxanes influence cell signalling beyond disrupting division can reveal mechanisms behind their side effects and improve therapeutic strategies.
- What they did: The study used in vitro reconstitution assays and microtubule structural analyses to show that taxanes and GMPCPP expand the microtubule lattice, inhibiting GEF-H1 binding and activating RhoA.
- The result: Findings suggest that microtubule lattice conformation controls key signalling pathways, providing insights into taxane action and potential avenues to mitigate adverse effects.