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De novo pan-rhodamine binders for fluorescence microscopy from mammalian cells to extremophiles.
Cell · · Journal Article
Chen, Yserentant + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Rhodamine binders (Rhobin) enable live- and fixed-cell fluorescence imaging with brightness surpassing existing tags, including reversible binding for STED and extended live-cell single-molecule imaging.
- Why it matters: Existing fluorescent tags lack a single system that combines high brightness, reversible binding, compact size, and stability across diverse conditions, limiting advanced microscopy and biosensing.
- What they did: The team de novo designed a pan-rhodamine binder using a generalizable strategy across diverse rhodamine wavelengths, then tested live/fixed imaging, STED, and live-cell single-molecule imaging versus HaloTag.
- The result: Rhobin supports super-resolution STED and extended-duration live-cell single-molecule imaging compared with HaloTag, and enables live imaging of Sulfolobus acidocaldarius at 75°C.
The findingWhy it mattersWhat they didThe result
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