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A Versatile Hairy Root Transformation System and Its Application in EjLEC2 Functional Research of Loquat.
Plant, Cell & Environment · · Journal Article
Wang, He + more
Abstract ↗AI summary
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An optimized Agrobacterium rhizogenes hairy-root transformation system in loquat (Eriobotrya japonica) enables functional testing of LEC2, revealing LEC2–FUS3 regulation of root growth.
- Why it matters: Loquat regeneration and transformation are difficult, limiting gene function studies of root growth and development and the roles of key regulators like LEC2.
- What they did: The study optimized conditions for rapid, stable hairy-root regeneration and transformation, then tested LEC2 and FUS3 via overexpression, loss-of-function mutants, and LEC2–FUS3 interaction assays.
- The result: LEC2 and FUS3 show a specific interaction with FUS3 acting downstream of LEC2; overexpression increased root length and root numbers, while mutants reduced them, and FUS3 overexpression compensated in LEC2 knockout mutants.
The findingWhy it mattersWhat they didThe result
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