Artificial metalloenzymes in complex biological environments.

Artificial metalloenzymes can be engineered to function effectively in complex biological environments, including cell lysates, surfaces, and intracellular spaces, overcoming previous limitations.

• Why it matters: This work addresses the challenge of cofactor inactivation and cellular stress caused by ArMs in biological systems, which has hindered their practical applications in medicine and biotechnology.
• What they did: The study reviews recent strategies for creating robust, biocompatible ArMs tailored for different biological contexts, analyzing their advantages, disadvantages, and environmental tolerances.
• The result: Advances in this field enable the development of more resilient ArMs with improved catalytic performance, opening new avenues for chemical synthesis, cellular control, and therapeutic interventions.