Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles.

An organelle-targeted DNA nanotrap detects norepinephrine (NE) with high selectivity and a ~50 ms response, enabling accurate NE imaging in organelles.

• Why it matters: Organelle-targeted neurotransmitter probes are lacking, limiting understanding of NE’s intracellular roles and how NE dynamics relate to neuronal injury outcomes.
• What they did: The team confined phenylboronic acid derivatives and hydroxymethyl groups into tetrahedral DNA nanostructures, added cyanine 3 for correction, and used a HaloTag ligand for organelle membrane targeting.
• The result: In organelle imaging, traumatic brain injury triggers NE bursts in the endoplasmic reticulum, linked to ER stress, altered ER–mitochondrial protein regulation, mitophagy, mitochondrial dysfunction, and neuronal death.

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