Non-coding RNA controls long-range enhancer-promoter interactions

A strong increase in enhancer-associated RNA molecules disrupts long-range enhancer–promoter interactions, while decreased RNA levels stabilize them, revealing RNA dosage control of 3D genome contacts.

• Why it matters: Long-range enhancer–promoter communication is crucial for transcription regulation, yet the molecular drivers of these distant contacts are not well defined.
• What they did: The study applied acute genetic and chemical perturbations of transcription elongation and termination, then integrated high-resolution 3D genome mapping with transcriptomics, proteomics, biochemistry, and live-cell super-resolution imaging.
• The result: RNA-mediated disruption of enhancer–promoter interactions is linked to reduced transcription of target genes, suggesting a regulatory feedback mechanism that depends on enhancer RNA levels.

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