The abstract is read at the publisher; the summary is JClub's.
In human EndoC-βH1 cells, 48-hour ER activation with PPT or DPN depolarizes resting membrane potential and increases firing frequency, while E2 and DPN reduce basal insulin secretion.
- Why it matters: ER and ERβ roles in endocrine pancreas physiology are established in animals, but how ER activation affects insulin-secreting human beta cells’ electrical activity and secretion remains unclear.
- What they did: EndoC-βH1 cells were treated for 48 hours with estradiol (E2), PPT, or DPN; insulin secretion was measured under non-stimulatory glucose, and patch-clamp recordings assessed membrane potential, firing, and ion currents.
- The result: PPT increased voltage-dependent Ca2+ channel currents, whereas DPN reduced K+ and Na+ currents; these treatments altered excitability without changing ion channel gene expression after 48 hours.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.