Identification and localization of bone marrow ILC2s using Id2/Cxcr6 dual-reporter mice

In Id2YFP/+ Cxcr6GFP/+ dual-reporter mice, bone marrow ILC2s are visualized as YFP+GFP+CD5−NKp46− cells distributed as isolated cells and multicellular clusters, with ILC2s comprising >90% of the reporter-positive population after exclusions.

• Why it matters: Bone marrow ILC2s are rare and share markers with other lymphoid cells, making their identification and spatial localization difficult.
• What they did: The study used spectral flow cytometry, single-cell RNA sequencing, and confocal microscopy in dual-reporter mice, applying CD25 and ICOS for enrichment and CD5 and NKp46 as exclusion markers.
• The result: The combined strategy supports that the in situ reporter-positive cells are putative ILC2s and enables mapping their spatial organization in bone marrow.

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