Women's health outreach using a rapid and fieldable Nanopore-based microbiome profiling workflow.

A rapid, fieldable Nanopore-based vaginal microbiome workflow at a three-day music festival in the Netherlands produced sequencing results within 4 hours using 16S rRNA profiling.

• Why it matters: Vaginal microbiome awareness is growing because Lactobacillus colonization is linked to protection against vaginal infectious diseases, yet practical tools for public engagement are limited.
• What they did: The team combined vaginal health dialogue, consent and questionnaires, self-collected swabs, QuickExtract DNA isolation, ultrafast 16S rRNA amplification with NextGenPCR and 16S Barcoding kit 24 V14, Nanopore R10 sequencing, and MABA16S analysis with modifications.
• The result: The workflow was completed and communicated to interested citizen-science participants within 4 hours, enabling rapid microbiome feedback alongside health promotion at Lowlands.

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