Putrescine-responsive post-transcriptional feedback regulation of Arabidopsis ADC2 mediated by 5'-UTR elements

Putrescine-responsive feedback repression of Arabidopsis thaliana ADC2 is mediated by its 5'-UTR, where AUU/CUG upstream ORF and ADC-box RNA structure are required.

• Why it matters: Plants must keep Put levels in a functional range, but the post-transcriptional mechanism linking Put abundance to ADC2 expression was unclear.
• What they did: Transient and transgenic assays used ADC2 5'-UTR:Eluc to test Put effects, while genome-edited deletions and cis-element mutations assessed which 5'-UTR features enable repression.
• The result: Exogenous Put reduced Eluc activity and translation efficiency without proportionate mRNA changes, and disrupting the AUU/CUG ORF or ADC-box abolished repression; 5'-UTR deletions caused delayed germination, impaired growth, and increased Put in the more severe line.

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