Direct Bioconjugations of Diverse Payloads using an Extraordinarily Donor-Promiscuous Flavin Transferase

ApbE, an extraordinarily donor-promiscuous flavin transferase, enables direct, site-specific enzymatic conjugation of diverse payloads using ADP-anchored donor substrates.

• Why it matters: Two-step enzyme-first/chemistry-second workflows often restrict payload diversity and require bioorthogonal handles, limiting efficiency and leaving molecular footprints.
• What they did: The study characterizes ApbE donor acceptance, showing it works with a broad set of ADP-anchored donors, then uses multiple donor-preparation strategies for direct ADDing.
• The result: Direct ADDing supports site-specific attachment of small molecules, peptides, proteins, and synthetic polymers, expanding donor generation options via naturally prevalent ADP-containing compounds.

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