Nuclear speckles comprise hierarchical nanoscale interaction networks that shape molecular dynamics

Nuclear speckles are hierarchically organized into dynamic nanoscale interaction networks, where proteins and RNAs alternate between diffusion and confinement within transient nanodomains.

• Why it matters: Condensates organize cellular processes, yet how their internal nanoscale architecture shapes molecular motion in living cells is unclear.
• What they did: Using MINFLUX nanoscopy with quantitative single-molecule imaging and live-cell tracking, they mapped macromolecule organization and dynamics in nuclear speckles and tested physiological and genetic perturbations.
• The result: Perturbations selectively remodeled molecular clustering and nanodomain organization without consistently changing component abundance, revealing multiscale condensate architecture with distinct but interconnected features.

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