NcSnRK1and NcRbohC1 regulate pollen germination on waterlily stigma after pollination

NcSnRK1 and NcRbohC1 regulate post-pollination pollen germination on waterlily stigma, with NcSnRK1 linked to activating NcRbohC1-dependent ROS burst in stigmatic papilla cells.

• Why it matters: The pre-fertilization barrier limits intersubgeneric hybridization in waterlily, and the mechanisms controlling pollen germination on the stigma after pollination remain unclear.
• What they did: The study used transient transgenic assays on waterlily stigma to test NcSnRK1 and NcRbohC1, and confirmed their interaction via yeast two-hybrid, luciferase complementation, and pull-down experiments.
• The result: Findings indicate NcSnRK1 may phosphorylate N-terminal sites of NcRbohC1 after pollination, activating ROS burst, disrupting ROS homeostasis on stigma, and inhibiting pollen germination.

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