Gentamicin impairs nephron development in iPSC-derived kidney organoids and disrupts proximal tubular lysosomal function

Continuous gentamicin exposure (0.25–1.5 mM) during iPSC-derived kidney organoid development (d7+18) impairs organoid growth and reduces nephron formation and maturation.

• Why it matters: Gentamicin nephrotoxicity affects about 10–20% of treated patients, and placental transfer raises concern for neonates and preterm infants during ongoing nephrogenesis.
• What they did: Human iPSC kidney organoids and magnetically sorted megalin-expressing proximal tubule cells were exposed to gentamicin, with proximal cells treated for 24 hours and intracellular congeners quantified by LC-MS/MS.
• The result: Gentamicin increases lipid accumulation and reduces lysosomal area in proximal tubule cells, suggesting disrupted metabolism and homeostasis during proximal tubular toxicity.

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