Detection of bifidobacterial lipoproteins with anti-viral potential in donor human milk

Donor human milk contains free bifidobacterial lipoproteins that cross a Caco-2 intestinal model within 4h while still binding CV B4, despite highly variable levels.

• Why it matters: Group B coxsackieviruses are linked to triggering type 1 diabetes, and free bifidobacterial lipoproteins can prevent viral cell infection.
• What they did: In a biobank of 90 donors, qPCR quantified Bifidobacterium breve and Bifidobacterium longum, while BLps were captured on CV B4, analyzed by SDS-PAGE and competitive ELISA, and tested for transport across Caco-2 monolayers.
• The result: BLps were readily detected, with B. breve in 93.0% and B. longum in 32.8% of unique samples; notably, free B. longum Lps appeared without B. longum itself.

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