bioRxivJClub
Alternative splicing switches human Musashi-2 between cytoplasmic and nuclear regulatory functions
bioRxiv · · Preprint · Open access
Walters, Koertel + 4 more
Abstract ↗AI summary
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Alternative 3' splicing in human MSI2 exon 12 toggles function by generating cytoplasmic MSI2-328 versus nuclear insertion-containing MSI2-324, with localization linked to translation-factor versus splicing-factor binding.
- Why it matters: MSI2 can both activate and repress translation, but those effects have been hard to attribute to cellular context alone.
- What they did: The study mapped an 18-amino-acid insertion that depends on an alternative 3' splice acceptor, then tested localization and reporter translation by tethering MSI2-328 or MSI2-324 to mRNAs and by removing the insertion.
- The result: The insertion is necessary and sufficient for nuclear localization, tethering MSI2-328 promotes translation while MSI2-324 does not, and the acceptor usage ranges from 19% in skeletal muscle to 51% in brain and increases in several tumor types.
The findingWhy it mattersWhat they didThe result
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