bioRxivJClub
Single-cell spatiotemporal transcriptomics reveals the origin, cellular heterogeneity and regulatory network of seed fiber cells in poplar
bioRxiv · · Preprint · Open access
Han, Wang + 6 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Single-nucleus RNA-seq and spatial transcriptomics in female poplar capsules traced seed fiber development, showing seed fibers originate from placenta epidermal cells and form three distinct subtypes.
- Why it matters: Molecular control of seed fiber (fluff) formation in poplar is poorly understood, limiting efforts to manage environmental and health concerns tied to these epidermal trichomes.
- What they did: The study integrated snRNA-seq with spatial transcriptomics to build a spatiotemporal atlas, then used developmental trajectory and network analysis to identify regulators including PtoPDF2, PtoMYB38, and PtoHDG11.
- The result: PtoPDF2 activates PtoMYB38 and PtoSTK2 by binding L1-box motifs, defining a module governing seed fiber initiation and providing targets for breeding low-fluff cultivars.
The findingWhy it mattersWhat they didThe result
- Open access