bioRxivJClub
Non-canonical peptidoglycan cross-linking is essential for Mycobacterium tuberculosis acid resistance
bioRxiv · · Preprint · Open access
Ma, Rea + 8 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
LdtB, an L,D transpeptidase in Mycobacterium tuberculosis, is essential for growth and survival under acid stress in a lipid-rich acidic medium that mimics the host environment.
- Why it matters: Acid resistance is required for Mycobacterium tuberculosis to survive and replicate inside macrophages, but the genetic determinants of this adaptation remain incompletely defined.
- What they did: A transposon screen in lipid-rich acidic medium identified ldtB; LdtB loss reduced 3-3 peptidoglycan cross-linking, disrupted cell wall architecture, impaired intrabacterial pH homeostasis, and increased susceptibility to cell wall-active antibiotics.
- The result: Mycobacterium tuberculosis lacking LdtB showed heightened sensitivity to meropenem within macrophages, supporting LdtB as a mediator linking peptidoglycan homeostasis to acid stress resistance and as a potential therapeutic target.
The findingWhy it mattersWhat they didThe result
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