Illuminating the Role of A-to-I Editing in Cancer using EndoVIA 2.0

EndoVIA 2.0 enables robust, spatial quantification of adenosine-to-inosine RNA editing in FFPE tissues, detecting ADAR1-dependent changes across multiple human models.

• Why it matters: Spatially mapping A-to-I editing in FFPE samples is difficult, limiting access to abundant archived clinical specimens where cancer-associated editing dysregulation may be measurable.
• What they did: The assay uses calcium-dependent immunostaining with human Endonuclease V as an affinity reagent, validated in ADAR1-altered cell lines, fresh frozen tissues, gastric organoids, and FFPE sections with quantitative imaging plus machine-learning segmentation.
• The result: In ADAR1 knockout and overexpression models, it resolves differential editing patterns and heterogeneity, distinguishes ADAR1-deficient from ADAR1-sufficient cells, and shows stage-dependent increases and subcellular localization shifts along the gastric neoplastic cascade.

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