The abstract is read at the publisher; the summary is JClub's.
A ketogenic diet increases proinflammatory macrophage accumulation across multiple mouse tissues, and long-chain saturated fatty acids drive this via cGAS–STING signaling.
- Why it matters: Ketogenic diets show mixed inflammatory effects, but the mechanism behind when they promote versus suppress inflammation is unclear.
- What they did: Using immunoblotting, immunohistochemistry, multiplex single-cell immunofluorescence, flow cytometry, and RNA sequencing, the study tested fatty-acid effects in murine and human macrophage cultures and profiled KD-fed mouse macrophages by metabolomics and transcriptomics.
- The result: Long-chain saturated fatty acids promoted M1-like polarization, enriched proinflammatory ceramides/hexosylceramides, and up-regulated inflammasome and cGAS–STING pathways; myeloid-lineage Sting knockout mice showed cGAS–STING is required for KD-driven proinflammatory effects.
The findingWhy it mattersWhat they didThe result
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