The abstract is read at the publisher; the summary is JClub's.
Epitope mapping of MPXV A35 identifies a conserved A35 dimer-interface epitope targeted by high-affinity epitope 3+4 mAb HD15, enabling broadly protective antibody combinations.
- Why it matters: With mpox virus infections since 2022 exceeding 188,000 reported cases globally by June 2026, there is a need for broadly effective therapeutics against orthopoxviruses.
- What they did: From donors with incidental vaccinia virus exposure, the team isolated anti-A33/A35 monoclonal antibodies using recombinant MPXV A35 as bait, mapped four nonoverlapping epitopes plus a composite epitope, and used cryo-electron microscopy to define HD15 binding.
- The result: Antibody pairs to nonoverlapping epitopes showed synergistic, Fc-dependent neutralization in vitro and conferred therapeutic protection in mice, reducing viral burden and disease severity after MPXV challenge.
The findingWhy it mattersWhat they didThe result
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