Catabolite activator protein and quorum sensing cross-control group behaviors in Vibrio campbellii.

CAP and LuxR co-occupied 11 promoters in Vibrio campbellii, co-regulating metabolism, respiration, and virulence, with the strongest co-regulation linked to luxCDABE.

• Why it matters: How nutrient sensing and quorum sensing transcription factors overlap to coordinate global behaviors in vibrios is understudied, limiting understanding of pathogenic lifestyle regulation.
• What they did: Using ChIP-seq and RNA-seq, the study mapped individual and combined CAP and LuxR regulons in Vibrio campbellii, including testing luxCDABE promoter binding and expression changes.
• The result: RNA-seq showed luxCDABE genes were the most strongly co-regulated, but ChIP-seq found only LuxR bound the luxCDABE promoter in vivo, implying CAP acts indirectly via an intermediate factor.

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