Nuclear TRiC states captured in situ.

Nuclear TRiC in human cells shows duty-cycle intermediates and AF3-modeled interaction surfaces, supporting compartment-specific roles in nuclear proteostasis.

• Why it matters: Cytosolic TRiC function is established, but nuclear TRiC is less understood, leaving a gap in how protein folding and proteostasis are managed inside the nucleus.
• What they did: The study combined cryo-electron tomography, cross-linking and affinity purification mass spectrometry, and AlphaFold3-based structural modeling to examine nuclear TRiC in human cells.
• The result: Nuclear TRiC intermediates were detected and multiple nuclear binding partners were identified, implying interaction sites on the chamber interior and enabling analysis of low-abundant events by cryo-ET.

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