Cell culture tightrope: Balancing amino acids and dipeptides to optimize media formulation and biomanufacturing performance.

Dipeptides are highlighted as an increasingly attractive alternative to free amino acids for CHO cell media, improving solubility and stability while supporting process performance.

• Why it matters: As mammalian bioprocessing intensifies and cell densities rise, amino acid delivery becomes constrained by stability and solubility limits, creating metabolic inefficiencies.
• What they did: The review synthesizes omics-assisted medium development, in silico thermodynamic models for amino acid–component interactions, and genetic engineering options for CHO cells.
• The result: Integrating experiments with modeling is presented as a smart media design path to predict solubility limits and optimize amino acid and dipeptide formulations for robust biomanufacturing.

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