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Bacterial 2',3'-cGAMP signaling induces membrane splitting in antiphage CBASS immunity.
Molecular Cell · · Journal Article
Li, Chen + more
Abstract ↗AI summary
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In the bacterial CdnB-Cap14 CBASS system, 2',3'-cGAMP signaling triggers Cap14 filament assembly that partitions the membrane and disrupts integrity, linked to abortive infection–associated cell death.
- Why it matters: The mechanism of how bacterial CBASS, related to mammalian cGAS-STING, converts 2',3'-cGAMP production into effective antiphage defense has been unclear.
- What they did: Using apo and ligand-bound structures of CdnB and Cap14, plus in vitro reconstitution, the authors show CdnB activation by DNA with intracellular ATP/GTP depletion to synthesize 2',3'-cGAMP, which drives Cap14 assembly from dimers into zipper-like two-layered filaments.
- The result: Cap14-driven membrane splitting supports abortive infection (Abi) protection against phage, while phage-encoded Acb2 sequesters 2',3'-cGAMP to block signaling, suggesting targets for therapeutic engineering.
The findingWhy it mattersWhat they didThe result
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