Identification of Vibrio cholerae proteins that bind 3',3'-cyclic guanosine adenine dinucleotide.

A 3',3'-cGAMP-binding screen of the Vibrio cholerae El Tor ORFeome identified seven additional 3',3'-cGAMP-binding proteins that also bind c-di-GMP with greater affinity.

• Why it matters: It is unclear whether horizontally acquired antiphage cyclic dinucleotide signals can interface with existing host signaling proteins and reshape cellular regulation after phage pressure.
• What they did: The authors screened the V. cholerae El Tor ORFeome for 3',3'-cGAMP binders, then tested whether expressing DncV, CapV, or both affected growth and swimming motility in V. cholerae and in heterologous bacteria.
• The result: DncV alone did not change growth or swimming motility, whereas DncV plus CapV reduced both in V. cholerae and in Escherichia coli, but Pseudomonas aeruginosa was less susceptible.

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