Nucleic Acids ResJClub
Queuosine-modification dependent histidyl tRNA fragment promotes cell-type dependent proliferation.
Nucleic Acids Research · · Journal Article
Zhang, Chew + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
In MCF7 breast cancer cells, QTRT1/2-dependent queuosine-modified tRNAs reduce proliferation, with tRFHis linked to lower translation efficiency of ribosomal proteins.
- Why it matters: tRFs regulate proliferation, but how queuosine modification and its dependence shape proliferation in specific mammalian cell types remains unclear.
- What they did: The study tested cell-type and Q-modification dependencies on proliferation, using QTRT1/2 dependence, transfection or knockdown of tRFHis, polysome profiling, and tRFHis pull-down to identify Msi2.
- The result: MCF7 proliferation decreased with Q-modified, QTRT1/2-dependent tRNAs; tRFHis transfection increased proliferation and its knockdown decreased it, while Msi2 knockdown reduced the Q- or tRFHis-dependent proliferation effects.
The findingWhy it mattersWhat they didThe result
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