Loss of splicing factor SR45 reshapes tissue-dependent alternative polyadenylation landscapes and transcriptome responses to salt in Arabidopsis.

Loss of the splicing factor SR45 in Arabidopsis thaliana reshapes tissue-specific alternative polyadenylation, with distal poly(A) site shifts occurring more often than proximal shifts.

• Why it matters: SR45 is known for alternative splicing, but its contribution to alternative polyadenylation—and how this affects salt-responsive transcriptomes—was unclear.
• What they did: Using PAT-seq and RNA-seq, the authors compared WT and sr45-1 across inflorescence, leaf, and root under normal conditions, and in leaf and root under prolonged mild salinity.
• The result: SR45 loss produced widespread APA changes with only partial overlap with AS and gene expression, and under salinity it altered distal versus proximal poly(A) usage, especially in leaf, alongside genotype-by-treatment differences in transcript abundance.

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