Nucleic Acids ResJClub
The Mycobacterium tuberculosis Ku C-terminus orchestrates LigD activity through domain-specific interactions.
Nucleic Acids Research · · Journal Article
Sowa, Huang + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
In Mycobacterium tuberculosis, the Ku C-terminus forms a bipartite interface with LigD that stimulates ligase activity while attenuating template-dependent polymerase activity.
- Why it matters: Ku-LigD complex function in NHEJ depends on Ku’s C-terminus, but how it coordinates multiple LigD activities was unclear, including potential species-specific differences.
- What they did: Using NMR spectroscopy, structural modelling, and mutational analysis, the study mapped Ku residues E246, V248, S258, K260, N266 and LigD residues D162, V194, R198 (polymerase) plus D522, K579, L580 (ligase).
- The result: Functional assays showed Ku stimulates LigD ligase activity via contacts with both polymerase and ligase domains and attenuates template-dependent polymerase activity; disrupting the interface abolishes stimulation and restores polymerase activity.
The findingWhy it mattersWhat they didThe result
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