Mol CellJClub
How the TREX-2 complex associates with the nuclear pore.
Molecular Cell · · Journal Article · Open access
Obarska-Kosinska, Zhu + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
An extended cryo-ET structure of the human nuclear pore complex shows TREX-2 core members GANP, Centrin-2, and ENY2 built into the nuclear ring, opposite NUP214.
- Why it matters: NPC architecture and how mRNP remodeling is structurally coupled to transport remain incompletely understood, especially for TREX-2’s role at the pore.
- What they did: The team combined cryo-electron tomography, crosslinking mass spectrometry, and AI-assisted integrative modeling to resolve TPR, NUP153, NUP50, and ZC3HC1 and identify five additional proteins.
- The result: TREX-2 is established as an integral NPC module rather than a transient factor, redefining the inner ring, nuclear ring, and nuclear basket and suggesting a structural basis for TREX-2-mediated mRNP remodeling linked to NPC transport.
The findingWhy it mattersWhat they didThe result
- Open access