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Waste sludge-derived humic acid alters metallic nanoparticle-bacteria interactions during denitrification via amide-mediated transformation and metabolic regulation.
Environmental research · · Journal Article
Ou, Wu + more
Abstract ↗AI summary
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Waste sludge-derived humic acid restores denitrification with 98.4% recovery versus 70.6% for natural analogs by transforming Ag nanoparticles via amide-mediated binding and metabolic regulation.
- Why it matters: Silver nanoparticles disrupt bacterial denitrification, a major driver of global nitrogen removal, and practical mitigation using scalable, sustainable materials is still limited.
- What they did: The study combined structural characterization with binding-energy measurements and transcriptomics to link WSHA’s amide-rich structure to Ag nanoparticle transformation and denitrification gene and pathway changes.
- The result: WSHA lowers the α-helix/(β-sheet+random coil) ratio to 0.31, strengthens Ag binding (-6.53 eV vs -5.41 eV), reduces bioavailable Ag+ release by 80%, and upregulates denitrification enzymes and stress responses.
The findingWhy it mattersWhat they didThe result
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