Establishment of a doxycycline-inducible p16(INK4a) knock-in human embryonic stem cell line, WAe009-A-3Y.

A doxycycline-inducible p16 INK4a knock-in human embryonic stem cell line was established, showing dose-dependent p16 INK4a and ZsGreen expression upon induction.

• Why it matters: Understanding p16 INK4a's role in cellular senescence is crucial for studying aging and age-related diseases, but suitable models are limited.
• What they did: Researchers targeted a Tet-On cassette into the AAVS1 locus of human embryonic stem cells, creating a line that maintains pluripotency, tri-lineage potential, and normal karyotype, with inducible p16 INK4a expression.
• The result: This cell line offers a valuable resource for developing p16 INK4a-related senescence models across various stem cell-derived lineages, enabling further aging research.